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pDonor NTRK1-P2A-tdTomato
(Plasmid #246653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246653 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2958
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P2A-tdTomato flanked by NTRK1 homology regions
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    tdTomato was altered to contain a silent SAL1 restriction site
  • Entrez Gene
    NTRK1 (a.k.a. MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ASCI (unknown if destroyed)
  • 3′ cloning site ASCI (unknown if destroyed)
  • 5′ sequencing primer M13 frw
  • 3′ sequencing primer M13 rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    P2A-tdTomato; cloned from HB9:P2A-TdT reporter construct (Garcia Diaz et al., 2020)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDonor NTRK1-P2A-tdTomato was a gift from Joriene de Nooij (Addgene plasmid # 246653 ; http://n2t.net/addgene:246653 ; RRID:Addgene_246653)

  • For your References section:

    Derivation and analysis of human somatic sensory neuron subtypes facilitated through fluorescent hPSC reporters. Malka-Gibor E, Oliver KM, Chen S, Garcia-Diaz A, Williams D, Henry JA, Akcan BT, Corneo B, de Nooij JC. Stem Cell Reports. 2026 Jan 2:102753. doi: 10.1016/j.stemcr.2025.102753. 10.1016/j.stemcr.2025.102753 PubMed 41483810
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