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pDonor MAFA-P2A-tdTomato
(Plasmid #246654)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246654 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2958
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P2A-tdTomato flanked by MAFA homology regions
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    MAFA (a.k.a. INSDM, RIPE3b1, hMafA)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    P2A-tdTomato; cloned from HB9:P2A-TdT reporter construct (Garcia Diaz et al., 2020)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDonor MAFA-P2A-tdTomato was a gift from Joriene de Nooij (Addgene plasmid # 246654 ; http://n2t.net/addgene:246654 ; RRID:Addgene_246654)

  • For your References section:

    Derivation and analysis of human somatic sensory neuron subtypes facilitated through fluorescent hPSC reporters. Malka-Gibor E, Oliver KM, Chen S, Garcia-Diaz A, Williams D, Henry JA, Akcan BT, Corneo B, de Nooij JC. Stem Cell Reports. 2026 Jan 2:102753. doi: 10.1016/j.stemcr.2025.102753. 10.1016/j.stemcr.2025.102753 PubMed 41483810
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