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pEGFP-C1-DNAJC13(2198t)
(Plasmid #246661)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246661 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFP-C1
  • Total vector size (bp) 11340
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNAJC13
  • Alt name
    RME-8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6592
  • Mutation
    Deletion of residues 2199 to 2243 (c-terminal residues)
  • Entrez Gene
    DNAJC13 (a.k.a. PARK21, RME8)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CGCGATCACATGGTCC
  • 3′ sequencing primer TCAGGGGGAGGTGTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-DNAJC13(2198t) was a gift from Braden Lobingier (Addgene plasmid # 246661 ; http://n2t.net/addgene:246661 ; RRID:Addgene_246661)

  • For your References section:

    DNAJC13 localization to endosomes is opposed by its J domain and its disordered C-terminus. Adoff H, Novy B, Holland E, Lobingier BT. Mol Biol Cell. 2025 Sep 1;36(9):ar114. doi: 10.1091/mbc.E24-12-0575. Epub 2025 Jul 30. 10.1091/mbc.E24-12-0575 PubMed 40737286
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