pSELIS-SRTF1
(Plasmid
#246681)
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PurposeUsed for growth-based enrichment of functional transcription factor variants and fluorescent-based readout of transcription factor function.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSELIS
- Total vector size (bp) 5984
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameSRTF1-responsive promoter
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Insert Size (bp)57
Gene/Insert 2
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Gene/Insert nameSRTF1-responsive promoter
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Insert Size (bp)57
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byDNA was ordered as a synthetic gene fragment.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains two SRTF1-responsive promoter sequences, one upstream of sfGFP and one upstream of a cI Repressor.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSELIS-SRTF1 was a gift from Andrew Ellington (Addgene plasmid # 246681 ; http://n2t.net/addgene:246681 ; RRID:Addgene_246681)
