pSELIS-SRTF1
(Plasmid #246681)

• Purpose: Used for growth-based enrichment of functional transcription factor variants and fluorescent-based readout of transcription factor function.
• Depositing Lab: Andrew Ellington
• Depositing Organization: University of Texas at Austin
  Located in the United States of America
• Publication: Example of directed evolution in E. coli to develop novel ligand-binding regulators: evolving a progesterone-responsive transcription factor to bind cortisol (unpublished)

Backbone

• Vector backbone: pSELIS
• Total vector size (bp): 5984
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: SRTF1-responsive promoter
• Insert Size (bp): 57

Gene/Insert 2

• Gene/Insert name: SRTF1-responsive promoter
• Insert Size (bp): 57

Resource Information

• A portion of this plasmid was derived from a plasmid made by: DNA was ordered as a synthetic gene fragment.

Terms and Licenses

• Academic/Nonprofit Terms:
  • Research Use Only Terms (Nonprofits)
• Industry Terms:
  • Research Use Only Terms (Industry)

Depositor Comments

This plasmid contains two SRTF1-responsive promoter sequences, one upstream of sfGFP and one upstream of a cI Repressor.