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p3xFLAG-CMV-9-sGPR124-VEGFR2 TMD-FKBP
(Plasmid #246697)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246697 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p3xFLAG-CMV-9
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPR124
  • Alt name
    ADGRA2
  • Alt name
    TEM5
  • Species
    H. sapiens (human)
  • Mutation
    ECD
  • Entrez Gene
    ADGRA2 (a.k.a. GPR124, TEM5)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • hVEGFR2 TMD-hFKBP1A F36V (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Bert Vogelstein (Addgene #16561)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-CMV-9-sGPR124-VEGFR2 TMD-FKBP was a gift from Calvin Kuo & Mario Vallon (Addgene plasmid # 246697 ; http://n2t.net/addgene:246697 ; RRID:Addgene_246697)

  • For your References section:

    A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific Regulation of Wnt Bioavailability. Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, Essler M, Noda M, Garcia KC, Kuo CJ. Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 10.1016/j.celrep.2018.09.045. 10.1016/j.celrep.2018.09.045 PubMed 30304675
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