Skip to main content
Addgene
Open Menu

p3xFLAG-CMV-9-CLIPf-GPR124
(Plasmid #246701)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246701 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    p3xFLAG-CMV-9
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPR124
  • Alt name
    ADGRA2
  • Alt name
    TEM5
  • Species
    H. sapiens (human)
  • Entrez Gene
    ADGRA2 (a.k.a. GPR124, TEM5)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG-CLIPf (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene sourced from cDNA clone FLJ00170

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-CMV-9-CLIPf-GPR124 was a gift from Calvin Kuo & Mario Vallon (Addgene plasmid # 246701 ; http://n2t.net/addgene:246701 ; RRID:Addgene_246701)

  • For your References section:

    A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific Regulation of Wnt Bioavailability. Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, Essler M, Noda M, Garcia KC, Kuo CJ. Cell Rep. 2018 Oct 9;25(2):339-349.e9. doi: 10.1016/j.celrep.2018.09.045. 10.1016/j.celrep.2018.09.045 PubMed 30304675
Related items: