pGL4.10 EXOC3prom Chr5-LG4
(Plasmid
#246712)
-
PurposeTo investigate the influence of an LG4 enhancer (chr5 LG4) on EXOC3 promoter activity (measured by luciferase expression)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepGL4.10
-
Vector typeLuciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameEXOC3 Promoter
-
SpeciesH. sapiens (human)
-
MutationWT
-
Entrez GeneEXOC3 (a.k.a. SEC6, SEC6L1, Sec6p)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Gene/Insert 2
-
Gene/Insert namechr5 LG4 enhancer
-
SpeciesH. sapiens (human)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGL4.10 EXOC3prom Chr5-LG4 was a gift from Glen Borchert (Addgene plasmid # 246712 ; http://n2t.net/addgene:246712 ; RRID:Addgene_246712) -
For your References section:
Long G4-rich enhancers target promoters via a G4 DNA-based mechanism. DeMeis JD, Roberts JT, Delcher HA, Godang NL, Coley AB, Brown CL, Shaw MH, Naaz S, Dahal A, Alqudah SY, Nguyen KN, Nguyen AD, Paudel SS, Shell JE, Patil SS, Dang H, O'Neal WK, Knowles MR, Houserova D, Gillespie MN, Borchert GM. Nucleic Acids Res. 2025 Jan 11;53(2):gkae1180. doi: 10.1093/nar/gkae1180. 10.1093/nar/gkae1180 PubMed 39658038