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pMDC32B-BS-AtMIR390a-A18G-B/c
(Plasmid #246716)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246716 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMDC32
  • Backbone size w/o insert (bp) 10133
  • Total vector size (bp) 11629
  • Vector type
    Plant Expression, RNAi
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Basal stem of Arabidopsis MIR390a precursor with A18G mutation & chloramphenicol-ccdB cassette flanked by two BsaI sites
  • Alt name
    BS-AtMIR390a-B/c
  • Alt name
    MIR390a
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1496
  • GenBank ID
    AT2G38325
  • Entrez Gene
    MIR390a (a.k.a. AT2G38325, MICRORNA 390, MIR390, MIR390A, microRNA390A, p_MI0001000)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDC32B-BS-AtMIR390a-A18G-B/c was a gift from Alberto Carbonell (Addgene plasmid # 246716 ; http://n2t.net/addgene:246716 ; RRID:Addgene_246716)

  • For your References section:

    Enhanced microRNA accumulation and gene silencing efficiency through optimized precursor base pairing. Llorens-Gamez JJ, Garcia-Cano PJ, Rico-Rodrigo S, Duyos-Casanova L, Toledano-Franco S, Carbonell A. Plant J. 2026 Jan;125(1):e70665. doi: 10.1111/tpj.70665. 10.1111/tpj.70665 PubMed 41505763
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