CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI
(Plasmid
#246757)
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PurposeRNA circularization with CIRC using Azo intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 246757 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepuc57-Kan
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCIRC-V1-Azo-CVB3 IRES-EGFP-BbsI
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI was a gift from Wensheng Wei (Addgene plasmid # 246757 ; http://n2t.net/addgene:246757 ; RRID:Addgene_246757) -
For your References section:
Self-splicing RNA circularization facilitated by intact group I and II introns. Shen Y, Li B, Dong L, Tang W, Ren J, Chen F, Zheng W, Yu Y, Gao L, Wei W. Nat Commun. 2025 Aug 10;16(1):7376. doi: 10.1038/s41467-025-62607-y. 10.1038/s41467-025-62607-y PubMed 40784880