EKAREN5-c
(Plasmid
#246761)
-
PurposeControl plasmid for EKAREN5 and EKAREN5-gl (original YPet - mTurquoise-GL pair)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246761 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepLV lentiviral vector
- Total vector size (bp) 10986
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEKAREN5-c
-
SpeciesAequorea victoria
-
Insert Size (bp)3429
-
Tag
/ Fusion Protein
- nls localization motif (C terminal on insert)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byHugo Snippert's lab (EKAREN5)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
EKAREN5-c was a gift from Toru Hiratsuka (Addgene plasmid # 246761 ; http://n2t.net/addgene:246761 ; RRID:Addgene_246761) -
For your References section:
A sensitive ERK fluorescent probe reveals the significance of minimal EGF-induced transcription. Weisheng Z, Nakayama J, Inomata Y, Higashiyama S, Hiratsuka T. Cell Struct Funct. 2024 Dec 18. doi: 10.1247/csf.24070. 10.1247/csf.24070 PubMed 39694501