pGGE-AtLDL1
(Plasmid
#246812)
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PurposeA GreenGate entry vector containing coding region of AtLDL1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepGGE000
- Backbone size w/o insert (bp) 2686
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Vector typeGreenGate cloning entry vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtLDL1
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2534
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Entrez GeneLDL1 (a.k.a. AT1G62830, ARABIDOPSIS LYSINE-SPECIFIC HISTONE DEMETHYLASE, ATLSD1, ATSWP1, F23N19.21, F23N19_21, LSD1, LSD1-like 1, LYSINE-SPECIFIC HISTONE DEMETHYLASE, SWP1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (not destroyed)
- 3′ cloning site BsaI (not destroyed)
- 5′ sequencing primer atgtgagttagctcactcattaggcac
- 3′ sequencing primer gcaaggcgattaagttgggtaacg
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGGE-AtLDL1 was a gift from Danhua Jiang (Addgene plasmid # 246812 ; http://n2t.net/addgene:246812 ; RRID:Addgene_246812)