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pGGF-NLS-NOS terminator-Hygromycin resistance
(Plasmid #246819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246819 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGGF000
  • Backbone size w/o insert (bp) 2686
  • Vector type
    GreenGate cloning entry vector
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-NOS terminator-Hygromycin resistance
  • Species
    Synthetic
  • Insert Size (bp)
    2651

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (not destroyed)
  • 3′ cloning site BsaI (not destroyed)
  • 5′ sequencing primer atgtgagttagctcactcattaggcac
  • 3′ sequencing primer gcaaggcgattaagttgggtaacg
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGF-NLS-NOS terminator-Hygromycin resistance was a gift from Danhua Jiang (Addgene plasmid # 246819 ; http://n2t.net/addgene:246819 ; RRID:Addgene_246819)
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