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pTwist_21tRNAs_split
(Plasmid #246822)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246822 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTwist vector
  • Backbone manufacturer
    Twist bioscience
  • Backbone size w/o insert (bp) 2959
  • Total vector size (bp) 5086
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E. coli tRNA arrays
  • Species
    E. coli
  • Insert Size (bp)
    2958

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTwist_21tRNAs_split was a gift from Norikazu Ichihashi (Addgene plasmid # 246822 ; http://n2t.net/addgene:246822 ; RRID:Addgene_246822)

  • For your References section:

    Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method. Miyachi R, Masuda K, Shimizu Y, Ichihashi N. Nat Commun. 2025 Aug 26;16(1):7418. doi: 10.1038/s41467-025-62588-y. 10.1038/s41467-025-62588-y PubMed 40858540
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