ARL13B-(ΔIDR)-GFP
(Plasmid
#246845)
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PurposeExpress Arl13B (resistance to sgRNA) with deletion of Intrinsically Disordered Region, tagged with GFP
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246845 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLVX-puro
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 8102
- Total vector size (bp) 9775
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameArl13B
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SpeciesH. sapiens (human)
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Insert Size (bp)997
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Mutationdeleted IDR domain
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Entrez GeneARL13B (a.k.a. ARL2L1, JBTS8)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ARL13B-(ΔIDR)-GFP was a gift from Lei Lu (Addgene plasmid # 246845 ; http://n2t.net/addgene:246845 ; RRID:Addgene_246845) -
For your References section:
Knocking out ARL13B completely abolishes primary ciliogenesis in cell lines. Mahajan D, Chia HM, Lu L. Sci Rep. 2025 Jul 24;15(1):26980. doi: 10.1038/s41598-025-11124-5. 10.1038/s41598-025-11124-5 PubMed 40707593