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pHR-BFP-NLS-P2A-RSV P-1xALFATag@72AA
(Plasmid #246864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246864 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pHR
  • Backbone size w/o insert (bp) 8271
  • Total vector size (bp) 9890
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BFP-NLS-P2A-RSV P-1xALFATag@72AA
  • Species
    RSV
  • Insert Size (bp)
    1620
  • Mutation
    RSV phosphoprotein (P) contains a single ALFA Tag inserted at amino acid 72.
  • Promoter SFFV
  • Tag / Fusion Protein
    • BFP-NLS (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.26.645422 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-BFP-NLS-P2A-RSV P-1xALFATag@72AA was a gift from Marvin Tanenbaum (Addgene plasmid # 246864 ; http://n2t.net/addgene:246864 ; RRID:Addgene_246864)

  • For your References section:

    Pre-assembly of biomolecular condensate seeds drives RSV replication. Ratnayake D, Galloux M, Boersma S, Noerenberg M, Sizun C, Sacristan C, Sourimant J, Lakerveld AJ, Gelderloos AT, Apperloo L, Demyanenko Y, Baars MJD, Banerjee R, Dreier B, Furler S, Mazur NI, Bont LJ, Mohammed S, Plückthun A, Éléouët J, Kops GJPL, Castello A, van Kasteren PB, Rameix-Welti M, Tanenbaum ME. Nature (2026). https://doi.org/10.1038/s41586-025-10071-5 10.1038/s41586-025-10071-5
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