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pAIDA_TEV_fap2EC
(Plasmid #246866)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246866 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAIDA1
  • Backbone manufacturer
    Gen Larsson, Addgene plasmid #79180
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    F. nucleatum ATCC23726 Fap2 (extracellular domain, res. 42–3271)
  • Alt name
    Fap2-EC
  • Species
    Fusobacterium nucleatum subsp. nucleatum ATCC23726
  • Insert Size (bp)
    9690
  • Tags / Fusion Proteins
    • 8x histidine (C terminal on backbone)
    • TEV protease cleavage site (C terminal on backbone)
    • Myc tag (C terminal on backbone)
    • AIDA autotransporter (C terminal on backbone)
    • AIDA signal sequence (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAIDA_TEV_fap2EC was a gift from Daniel Roderer (Addgene plasmid # 246866 ; http://n2t.net/addgene:246866 ; RRID:Addgene_246866)

  • For your References section:

    Structural basis of Fusobacterium nucleatum adhesin Fap2 interaction with receptors on cancer and immune cells. Schopf F, Marongiu GL, Milaj K, Sprink T, Kikhney J, Moter A, Roderer D. Nat Commun. 2025 Aug 29;16(1):8104. doi: 10.1038/s41467-025-63451-w. 10.1038/s41467-025-63451-w PubMed 40883327
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