pRSETA-mScarlet3-S2
(Plasmid
#246871)
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PurposeExpression of mScarlet3-S2
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246871 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepRSRTA
- Backbone size w/o insert (bp) 2872
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemScarlet3-S2
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SpeciesSynthetic
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Insert Size (bp)687
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH_ (not destroyed)
- 3′ cloning site EcoR_ (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7t
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSETA-mScarlet3-S2 was a gift from Pingyong Xu (Addgene plasmid # 246871 ; http://n2t.net/addgene:246871 ; RRID:Addgene_246871) -
For your References section:
A highly photostable monomeric red fluorescent protein for dual-color 3D STED and time-lapse 3D SIM imaging. Ding Y, He W, Wang K, Xue F, Zheng K, Zhao S, Qi D, Li T, Xia P, Pan L, Chen L, Chen Z, Yuan L, Xu P. Nat Methods. 2025 Nov 26. doi: 10.1038/s41592-025-02962-2. 10.1038/s41592-025-02962-2 PubMed 41299071