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mScarlet3-S2-Lamin A/C
(Plasmid #246875)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246875 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 3962
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mScarlet3-S2
  • Species
    Synthetic
  • Insert Size (bp)
    687
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe_ (not destroyed)
  • 3′ cloning site Bgl_ (not destroyed)
  • 5′ sequencing primer CMV-Forward
  • 3′ sequencing primer SV40 polyA-Reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Lamin A/C sequence was fused in-frame with mScarlet3-S2 and inserted into the Nhe_/Bgl_ sites of pEGFP-N1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mScarlet3-S2-Lamin A/C was a gift from Pingyong Xu (Addgene plasmid # 246875 ; http://n2t.net/addgene:246875 ; RRID:Addgene_246875)

  • For your References section:

    A highly photostable monomeric red fluorescent protein for dual-color 3D STED and time-lapse 3D SIM imaging. Ding Y, He W, Wang K, Xue F, Zheng K, Zhao S, Qi D, Li T, Xia P, Pan L, Chen L, Chen Z, Yuan L, Xu P. Nat Methods. 2025 Nov 26. doi: 10.1038/s41592-025-02962-2. 10.1038/s41592-025-02962-2 PubMed 41299071
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