DBJS-p9.6
(Plasmid
#246903)
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PurposeUsed for multi-site piggybac transposon insertion of CRY2-tagged G3BP2 for overexpression
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 6613
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRY2::G3BP2 (no dimerization domain) ::OLLAS::eGFP transposon + Puro resistance
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SpeciesH. sapiens (human)
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Entrez GeneG3BP2
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unspecified (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DBJS-p9.6 was a gift from David Bartel (Addgene plasmid # 246903 ; http://n2t.net/addgene:246903 ; RRID:Addgene_246903)