UBC-CCDC134-HA-hygro
(Plasmid
#246904)
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PurposeExpresses HA-tagged human CCDC134 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246904 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonelentiviral expression plasmid
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCCDC134
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SpeciesH. sapiens (human)
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Insert Size (bp)687
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Entrez GeneCCDC134 (a.k.a. OI22)
- Promoter UBC
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UBC-CCDC134-HA-hygro was a gift from William Kaelin (Addgene plasmid # 246904 ; http://n2t.net/addgene:246904 ; RRID:Addgene_246904) -
For your References section:
Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-kappaB. Lampson BL, Ramiotarez AS, Baro M, He L, Hegde M, Koduri V, Pfaff JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, Locher KP, Kaelin WG Jr. Cell. 2024 Apr 25;187(9):2209-2223.e16. doi: 10.1016/j.cell.2024.03.022. 10.1016/j.cell.2024.03.022 PubMed 38670073