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pPIC9K_A2AR_316_V229C_R291A
(Plasmid #246917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246917 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pPIC9K
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Adenosine A2A receptor
  • Species
    H. sapiens (human)
  • Mutation
    truncated to aa 1-316, V229C, R291A
  • Entrez Gene
    ADORA2A (a.k.a. A2aR, ADORA2, RDC8)
  • Promoter AOX1
  • Tags / Fusion Proteins
    • alpha-factor secretion signal, FLAG (N terminal on insert)
    • 10xHis (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPIC9K_A2AR_316_V229C_R291A was a gift from Libin Ye (Addgene plasmid # 246917 ; http://n2t.net/addgene:246917 ; RRID:Addgene_246917)

  • For your References section:

    Intermediate-state-trapped mutants pinpoint G protein-coupled receptor conformational allostery. Wang X, Neale C, Kim SK, Goddard WA, Ye L. Nat Commun. 2023 Mar 10;14(1):1325. doi: 10.1038/s41467-023-36971-6. 10.1038/s41467-023-36971-6 PubMed 36899002
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