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pCAG-mcherry-Tet1CDmut (pc2815)
(Plasmid #246923)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246923 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAG-IRES
  • Total vector size (bp) 9034
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tet1
  • Species
    M. musculus (mouse)
  • Mutation
    aa1365-2057 / H1652Y, D1654A
  • Entrez Gene
    Tet1 (a.k.a. 2510010B09Rik, Cxxc6, D10Ertd17e, LCX, mKIAA1676)
  • Promoter CAG
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AsiSI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-mcherry-Tet1CDmut (pc2815) was a gift from Cristina Cardoso (Addgene plasmid # 246923 ; http://n2t.net/addgene:246923 ; RRID:Addgene_246923)

  • For your References section:

    Binding of MBD proteins to DNA blocks Tet1 function thereby modulating transcriptional noise. Ludwig AK, Zhang P, Hastert FD, Meyer S, Rausch C, Herce HD, Muller U, Lehmkuhl A, Hellmann I, Trummer C, Storm C, Leonhardt H, Cardoso MC. Nucleic Acids Res. 2017 Mar 17;45(5):2438-2457. doi: 10.1093/nar/gkw1197. 10.1093/nar/gkw1197 PubMed 27923996
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