phU6-LaTranC-sgRNA
(Plasmid
#246933)
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Purposefor HEK293T human cell genome editing
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246933 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonephU6
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLaTranC sgRNA
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gRNA/shRNA sequenceUnspecified
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
phU6-LaTranC-sgRNA was a gift from Caixia Gao (Addgene plasmid # 246933 ; http://n2t.net/addgene:246933 ; RRID:Addgene_246933) -
For your References section:
Functional RNA splitting drove the evolutionary emergence of type V CRISPR-Cas systems from transposons. Jin S, Zhu Z, Li Y, Zhang S, Liu Y, Li D, Li Y, Luo Y, Cheng Z, Zhao KT, Gao Q, Yang G, Li H, Liang R, Zhang R, Qiu JL, Zhang YE, Liu JG, Gao C. Cell. 2025 Sep 29:S0092-8674(25)01035-9. doi: 10.1016/j.cell.2025.09.004. 10.1016/j.cell.2025.09.004 PubMed 41027434