Skip to main content
Addgene
Open Menu

pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis
(Plasmid #246980)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246980 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pET28a(+)v2
  • Backbone manufacturer
    NA
  • Backbone size w/o insert (bp) 5209
  • Total vector size (bp) 7081
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FLP Recombinase
  • Alt name
    FLP1
  • Alt name
    FLP3
  • Alt name
    Flippase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1872
  • GenBank ID
    AAB59340.1
  • Promoter T7
  • Tags / Fusion Proteins
    • TwinStrep (N terminal on insert)
    • SUMO (N terminal on insert)
    • SV40 (N terminal on insert)
    • Nucleoplasmin (C terminal on insert)
    • 8xHis (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The original gene sequence for FLP Recombinase was obtained from Addgene Plasmid #45978. The sequence and all tags were codon optimized for E. coli expression and synthesized by Twist Bioscience. The original backbone sequence for this modified pET28a(+) was obtained from Addgene Plasmid #154464.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We use a tag-free SUMO protease (ulp1) for cleaving the TwinStrep tag from the final product. Tag-free ulp1 can be synthesized using Addgene Plasmid #190063 and a His-tagged TEV protease or HRV-3C protease (PreScission protease).

The total insert size displayed on this page (1872bp) includes all tags and linkers.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis was a gift from Amro Hamdoun (Addgene plasmid # 246980 ; http://n2t.net/addgene:246980 ; RRID:Addgene_246980)
Related items: