pscAAV- EF1α core -hKCNJ2-V77E-HA tag
(Plasmid
#246996)
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PurposeCan be used to generate AAV virus that will express human KCNJ2 V77E mutant with Ala115 HA tag from EF1α core promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAAV with one trs deleted ITR and CAG promoter
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKCNJ2
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SpeciesH. sapiens (human)
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Insert Size (bp)1284
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MutationV77E
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GenBank IDNM_000891.3
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Entrez GeneKCNJ2 (a.k.a. ATFB9, HHBIRK1, HHIRK1, IRK1, KIR2.1, LQT7, SQT3)
- Promoter EF1α
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Tag
/ Fusion Protein
- HA tag (Internal at Ala115)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pscAAV- EF1α core -hKCNJ2-V77E-HA tag was a gift from Ting Chen (Addgene plasmid # 246996 ; http://n2t.net/addgene:246996 ; RRID:Addgene_246996) -
For your References section:
Fibroblast bioelectric signaling drives hair growth. Chen D, Yu Z, Wu W, Du Y, Du Q, Huang H, Li Y, Xuan T, Liang YC, Liu Y, Wang Z, Su R, Zhao Y, Li Q, Luo M, Wang F, Li J, Chuong CM, Lin Z, Chen T. Cell. 2025 Aug 14:S0092-8674(25)00857-8. doi: 10.1016/j.cell.2025.07.035. 10.1016/j.cell.2025.07.035 PubMed 40818454