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pFRT-B-GPCNA (pc1274)
(Plasmid #247001)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247001 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pFRT-lacZeo
  • Total vector size (bp) 6751
  • Vector type
    Cre/Lox
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PCNA
  • Species
    H. sapiens (human)
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Promoter T7
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT-B-GPCNA (pc1274) was a gift from Cristina Cardoso (Addgene plasmid # 247001 ; http://n2t.net/addgene:247001 ; RRID:Addgene_247001)

  • For your References section:

    4D Visualization of replication foci in mammalian cells corresponding to individual replicons. Chagin VO, Casas-Delucchi CS, Reinhart M, Schermelleh L, Markaki Y, Maiser A, Bolius JJ, Bensimon A, Fillies M, Domaing P, Rozanov YM, Leonhardt H, Cardoso MC. Nat Commun. 2016 Apr 7;7:11231. doi: 10.1038/ncomms11231. 10.1038/ncomms11231 PubMed 27052570
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