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pHCFnc/V5
(Plasmid #247003)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247003 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1/Hygro
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 11705
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HCF-1
  • Alt name
    HCFC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6105
  • Mutation
    Glu to Ala change at amino acid positions 1019,1081,110,1295,1323,1358,1423 that result in non cleavable protein
  • GenBank ID
    NM_005334.3
  • Entrez Gene
    HCFC1 (a.k.a. CFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3)
  • Tag / Fusion Protein
    • V5 tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCFnc/V5 was a gift from Thomas Kristie (Addgene plasmid # 247003 ; http://n2t.net/addgene:247003 ; RRID:Addgene_247003)

  • For your References section:

    Site-specific proteolysis of the transcriptional coactivator HCF-1 can regulate its interaction with protein cofactors. Vogel JL, Kristie TM. Proc Natl Acad Sci U S A. 2006 May 2;103(18):6817-22. doi: 10.1073/pnas.0602109103. Epub 2006 Apr 19. 10.1073/pnas.0602109103 PubMed 16624878
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