pFB1.HMBP-Prs.AEBP2iso2.mt2∆
(Plasmid
#247011)
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PurposeExpresses human AEBP2iso2.mt2∆ in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247011 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR)
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Gentamicin, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAEBP2iso2.mt2∆
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SpeciesH. sapiens (human)
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Mutationdeletion of aa 95-109
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Entrez GeneAEBP2
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- 6xHis-MBP (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFB1.HMBP-Prs.AEBP2iso2.mt2∆ was a gift from Chen Davidovich (Addgene plasmid # 247011 ; http://n2t.net/addgene:247011 ; RRID:Addgene_247011)