pBH001 (TadDE-T7RNAP)
(Plasmid
#247050)
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PurposeExpresses the fusion of TadDE (dual deaminase) and T7RNAP for gene-specific mutagenesis
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247050 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBAD33
- Backbone size w/o insert (bp) 5352
- Total vector size (bp) 8857
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsExpression for gene-specific mutagenesis in E. coli strain W3110
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTadDE-T7RNAP
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SpeciesSynthetic
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Insert Size (bp)3641
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Entrez GeneT7p07 (a.k.a. T7p07)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBH001 (TadDE-T7RNAP) was a gift from Seokhee Kim (Addgene plasmid # 247050 ; http://n2t.net/addgene:247050 ; RRID:Addgene_247050)