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J23100_PheS*_codon
(Plasmid #247070)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247070 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    N/A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mutated (A294G) E.coli PheS of which codon is changed
  • Alt name
    pheS
  • Alt name
    phenylalanyl-tRNA synthetase
  • Species
    Escherichia coli K-12 W3110

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory. Addgene NGS finds a frameshift mutation in LacI at amino acid #329 (WP_000248563.1). The depositing lab confirms this change is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    J23100_PheS*_codon was a gift from Seokhee Kim (Addgene plasmid # 247070 ; http://n2t.net/addgene:247070 ; RRID:Addgene_247070)

  • For your References section:

    Continuous targeted hypermutation with a tunable mutation window. Lee C, Kim S. Nat Commun. 2025 Nov 25. doi: 10.1038/s41467-025-66736-2. 10.1038/s41467-025-66736-2 PubMed 41290661
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