pSXL101
(Plasmid
#247078)
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PurposepZE12-luc containing MNX1 from Candida parapsilosis CBS604 and ubiC from E. coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepZE12-luc
- Backbone size w/o insert (bp) 2271
- Total vector size (bp) 5619
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameMNX1
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SpeciesE.coli
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Insert Size (bp)1437
Gene/Insert 2
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Gene/Insert nameubiC
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SpeciesE.coli
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Insert Size (bp)498
Gene/Insert 3
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Gene/Insert nameAS
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SpeciesE.coli
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Insert Size (bp)1411
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Addgene NGS finds a 3 nucleotide insertion in MNX1 that adds Thr at aa 51 compared to the reference sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSXL101 was a gift from Yajun Yan (Addgene plasmid # 247078 ; http://n2t.net/addgene:247078 ; RRID:Addgene_247078) -
For your References section:
High-level De novo biosynthesis of arbutin in engineered Escherichia coli. Shen X, Wang J, Wang J, Chen Z, Yuan Q, Yan Y. Metab Eng. 2017 Jul;42:52-58. doi: 10.1016/j.ymben.2017.06.001. Epub 2017 Jun 2. 10.1016/j.ymben.2017.06.001 PubMed 28583673