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pSXL101
(Plasmid #247078)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247078 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pZE12-luc
  • Backbone size w/o insert (bp) 2271
  • Total vector size (bp) 5619
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    MNX1
  • Species
    E.coli
  • Insert Size (bp)
    1437

Gene/Insert 2

  • Gene/Insert name
    ubiC
  • Species
    E.coli
  • Insert Size (bp)
    498

Gene/Insert 3

  • Gene/Insert name
    AS
  • Species
    E.coli
  • Insert Size (bp)
    1411

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Addgene NGS finds a 3 nucleotide insertion in MNX1 that adds Thr at aa 51 compared to the reference sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSXL101 was a gift from Yajun Yan (Addgene plasmid # 247078 ; http://n2t.net/addgene:247078 ; RRID:Addgene_247078)
  • For your References section:

    High-level De novo biosynthesis of arbutin in engineered Escherichia coli. Shen X, Wang J, Wang J, Chen Z, Yuan Q, Yan Y. Metab Eng. 2017 Jul;42:52-58. doi: 10.1016/j.ymben.2017.06.001. Epub 2017 Jun 2. 10.1016/j.ymben.2017.06.001 PubMed 28583673