K48R FLAG-HaloTag-RTEL1_DonorVector
(Plasmid
#247171)
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PurposeHR Donor for K48R C-terminal FLAG-HaloTag-RTEL1 fusion in human cells. Includes Puro selection marker.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247171 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC19
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameK48R FLAG-HaloTag-RTEL1
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SpeciesH. sapiens (human)
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Entrez GeneRTEL1 (a.k.a. C20orf41, DKCA4, DKCB5, NHL, PFBMFT3, RTEL)
- Promoter Endogenous RTEL1 promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
K48R FLAG-HaloTag-RTEL1_DonorVector was a gift from Thomas Cech (Addgene plasmid # 247171 ; http://n2t.net/addgene:247171 ; RRID:Addgene_247171) -
For your References section:
Rapid dynamics allow the low-abundance RTEL1 helicase to promote telomere replication. Wu G, Taylor E, Youmans DT, Arnoult N, Cech TR. Nucleic Acids Res. 2025 Feb 27;53(5):gkaf177. doi: 10.1093/nar/gkaf177. 10.1093/nar/gkaf177 PubMed 40087886