p16-GFP-CYRI-A
(Plasmid #247175)

• Purpose: Expresses recombinant fluorescent CYRI-A (Fam49A) protein in mammalian cells, specifically mouse Cyri-A with an internal GFP after proline 16, preserving the proposed N-terminal myristoylation site.
• Depositing Lab: Laura Machesky
• Publication: Le et al J Cell Biol. 2021 Sep 6;220(9). pii: 212437. doi: 10.1083/jcb.202012114. Epub 2021 Jun 24.

Backbone

• Vector backbone: pcDNA3.1(+)
• Total vector size (bp): 7051
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cyri-A
• Alt name: Fam49A
• Species: M. musculus (mouse)
• Entrez Gene: Cyria (a.k.a. 2410157M17Rik, 9630047E15, D12Ertd553e, Fam49a)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • P16-GFP-Cyri-A.gb
• A portion of this plasmid was derived from a plasmid made by: This was cloned in Laura Machesky's Lab by Anh Hoang Le, at the CRUK Scotland Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Laura Machesky's laboratory is now in the Department of Biochemistry at the University of Cambridge