pSBtet-GP-α+δ
(Plasmid
#247177)
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PurposeFor generating Sleeping Beauty-based stable cell line expressing human muscle acetylcholine receptor alpha1 and delta subunits. Expresses GFP; puromycin selection.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSBtet-GP
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuman muscle acetylcholine receptor alpha1 and delta subunits
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Alt nameCHRNA1 and CHRND
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- Twin strep tag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSBtet-GP-α+δ was a gift from Ryan Hibbs (Addgene plasmid # 247177 ; http://n2t.net/addgene:247177 ; RRID:Addgene_247177) -
For your References section:
Autoimmune mechanisms elucidated through muscle acetylcholine receptor structures. Li H, Pham MC, Teng J, O'Connor KC, Noviello CM, Hibbs RE. Cell. 2025 May 1;188(9):2390-2406.e20. doi: 10.1016/j.cell.2025.03.004. Epub 2025 Apr 8. 10.1016/j.cell.2025.03.004 PubMed 40203823