pSBtet-RH-β+ε
              
              
                (Plasmid
                
                #247178)
              
            
            
            
          - 
            PurposeFor generating Sleeping Beauty-based stable cell line expressing human muscle acetylcholine receptor beta1 and epsilon subunits. Expresses RFP; hygromycin selection.
 - 
              Depositing Lab
 - 
          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247178 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
- 
            Vector backbonepSBtet-RH
 - 
              Vector typeMammalian Expression
 - 
                Selectable markersHygromycin
 
Growth in Bacteria
- 
            Bacterial Resistance(s)Ampicillin, 100 μg/mL
 - 
            Growth Temperature37°C
 - 
            Growth Strain(s)DH5alpha
 - 
            Copy numberUnknown
 
Gene/Insert
- 
                Gene/Insert nameHuman muscle acetylcholine receptor beta and epsilon subunits
 - 
                  Alt nameCHRNB1 and CHRNE
 - 
                    SpeciesH. sapiens (human)
 - 
    
        Tag
        / Fusion Protein
    
- 3xFLAG tag
 
 
Resource Information
- 
            
            
            Supplemental Documents
 
Terms and Licenses
- 
        Academic/Nonprofit Terms
 - 
      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
- 
              
For your Materials & Methods section:
pSBtet-RH-β+ε was a gift from Ryan Hibbs (Addgene plasmid # 247178 ; http://n2t.net/addgene:247178 ; RRID:Addgene_247178) - 
                
For your References section:
Autoimmune mechanisms elucidated through muscle acetylcholine receptor structures. Li H, Pham MC, Teng J, O'Connor KC, Noviello CM, Hibbs RE. Cell. 2025 May 1;188(9):2390-2406.e20. doi: 10.1016/j.cell.2025.03.004. Epub 2025 Apr 8. 10.1016/j.cell.2025.03.004 PubMed 40203823