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pcDNA5-ZNF207-ZnF+Hel_K42R_3xFLAG
(Plasmid #247233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247233 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin, only when integrated into FlpIn FRT site

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZNF207-ZnF+Hel_K42R
  • Alt name
    BuGZ
  • Species
    H. sapiens (human)
  • Entrez Gene
    ZNF207 (a.k.a. BuGZ, hBuGZ)
  • Promoter CMV/TO inducible promoter
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.04.25.648738 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-ZNF207-ZnF+Hel_K42R_3xFLAG was a gift from Thomas Gonatopoulos-Pournatzis (Addgene plasmid # 247233 ; http://n2t.net/addgene:247233 ; RRID:Addgene_247233)

  • For your References section:

    RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeria. Behera AK, Kim JJ, Kordale S, Pekovic F, Damodaran AP, Kumari B, Vidak S, Dickson E, Xiao MS, Duncan G, Andresson T, Misteli T, Valkov E, Gonatopoulos-Pournatzis T. Mol Cell. 2026 Jan 8;86(1):41-59.e15. doi: 10.1016/j.molcel.2025.12.003. Epub 2025 Dec 30. 10.1016/j.molcel.2025.12.003 PubMed 41475346
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