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pNP3386 [For use in genome insertion and off-target assessment]
(Plasmid #247258)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247258 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    N/A
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.14.653916 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNP3386 [For use in genome insertion and off-target assessment] was a gift from Patrick Hsu (Addgene plasmid # 247258 ; http://n2t.net/addgene:247258 ; RRID:Addgene_247258)

  • For your References section:

    Megabase-scale human genome rearrangement with programmable bridge recombinases. Perry NT, Bartie LJ, Katrekar D, Gonzalez GA, Durrant MG, Pai JJ, Fanton A, Martins JQ, Hiraizumi M, Ricci-Tam C, Nishimasu H, Konermann S, Hsu PD. Science. 2025 Sep 25:eadz0276. doi: 10.1126/science.adz0276. 10.1126/science.adz0276 PubMed 40997214
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