NSK465 SFFV-mCherry-SGc(os13.NOT-SCN1A_20N.45,64,45.lowBG.12bp loop)cSG-EGFP-(3xMS2) (FLP-IN)
(Plasmid
#247290)
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PurposeLow baseline modulADAR sensor for NOT SCN1a exon 20N
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247290 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneFLP-IN
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-os13.NOT-SCN1A_20N.45,64,45.lowBG.12bp loop-EGFP
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SpeciesSynthetic
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MutationWT
- Promoter SFFV
Cloning Information
- Cloning method Unknown
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NSK465 SFFV-mCherry-SGc(os13.NOT-SCN1A_20N.45,64,45.lowBG.12bp loop)cSG-EGFP-(3xMS2) (FLP-IN) was a gift from Xiaojing Gao (Addgene plasmid # 247290 ; http://n2t.net/addgene:247290 ; RRID:Addgene_247290) -
For your References section:
Sequence-Independent RNA Sensing in Living Mammalian Cells. Kolber NS, K. Kaseniit E, Lanz TV, Robinson WH, Gao XJ. bioRxiv 2025.09.18.677213 10.1101/2025.09.18.677213