pUBC-SiT-EGFP-MS2
(Plasmid
#247294)
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PurposeExpresses a secretome mRNA reporter: MS2 mRNA encoding 45aa of the Type II membrane protein, sialyltransferase (a Golgi enzyme), fused with EGFP.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247294 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUBC
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSiT-EGFP
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SpeciesH. sapiens (human)
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Insert Size (bp)3355
- Promoter UbC
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUBC-SiT-EGFP-MS2 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 247294 ; http://n2t.net/addgene:247294 ; RRID:Addgene_247294) -
For your References section:
Secretome translation shaped by lysosomes and lunapark-marked ER junctions. Choi H, Liao YC, Yoon YJ, Grimm J, Wang N, Lavis LD, Singer RH, Lippincott-Schwartz J. Nature. 2025 Nov 5. doi: 10.1038/s41586-025-09718-0. 10.1038/s41586-025-09718-0 PubMed 41193816
