pPB-CrPV-IRES-cytERM-SUNTAG-MS2
(Plasmid
#247299)
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PurposeMammalian expression of ER-specific SUNTAG reporter: piggyBac vector expressing cytERM-SUNTAG MS2; 5’ UTR includes cricket paralysis virus IRES, allowing escape from translation initiation regulation.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247299 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepPB
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePB-CrPV-IRES-cytERM-SUNTAG-MS2
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-CrPV-IRES-cytERM-SUNTAG-MS2 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 247299 ; http://n2t.net/addgene:247299 ; RRID:Addgene_247299) -
For your References section:
Secretome translation shaped by lysosomes and lunapark-marked ER junctions. Choi H, Liao YC, Yoon YJ, Grimm J, Wang N, Lavis LD, Singer RH, Lippincott-Schwartz J. Nature. 2025 Nov 5. doi: 10.1038/s41586-025-09718-0. 10.1038/s41586-025-09718-0 PubMed 41193816
