pCMV-L10A-Halo
(Plasmid
#247300)
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PurposeMammalian expression of the large ribosomal submit protein L10A tagged with HaloTag; for single-molecule tracking of ribosomes.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUBC
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepUBC-L10A-Halo
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-L10A-Halo was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 247300 ; http://n2t.net/addgene:247300 ; RRID:Addgene_247300) -
For your References section:
Secretome translation shaped by lysosomes and lunapark-marked ER junctions. Choi H, Liao YC, Yoon YJ, Grimm J, Wang N, Lavis LD, Singer RH, Lippincott-Schwartz J. Nature. 2025 Nov 5. doi: 10.1038/s41586-025-09718-0. 10.1038/s41586-025-09718-0 PubMed 41193816