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pUBC_IGF2BP2(IMP2)_serinemod_E488QADAR_p2A_yGFP
(Plasmid #247301)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247301 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP (Addgene Plasmid #154786)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Adenosine deaminse acting on RNA
  • Alt name
    ADAR
  • Species
    D. melanogaster (fly), Synthetic
  • Insert Size (bp)
    1281
  • Mutation
    E488Q, hyperactive variant
  • Entrez Gene
    Adar (a.k.a. Dmel_CG12598, ADAR, ADAR1, CG12598, Dmel\CG12598, EG:BACN35H14.1, adar, adr, cg12598, dADAR, dAdar, dadar, hypnos-2)
  • Promoter UBC
  • Tags / Fusion Proteins
    • GFP (C terminal on insert)
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site PshA1 (not destroyed)
  • 5′ sequencing primer UBC_F (5'-CGCCGATGATTATATAAGGA-3')
  • 3′ sequencing primer GFP_R (5'-GCATCACCTTCACCTTCACC-3')
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUBC_IGF2BP2(IMP2)_serinemod_E488QADAR_p2A_yGFP was a gift from Robert Singer (Addgene plasmid # 247301 ; http://n2t.net/addgene:247301 ; RRID:Addgene_247301)

  • For your References section:

    HyperTRIBE identifies hepatic IGF2BP2/IMP2 targets in vivo and links IMP2 to autophagy. Do HTT, Both S, Kröhler T, Pirritano M, Wonterghem EV, Raab E, Ahne L, Franzenburg S, Ibrahim EM, Simon M, Biswas J, Helms V, Kessler SM, Kiemer AK. NAR Mol Med, 2026 10.1093/narmme/ugag034
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