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pLN053
(Plasmid #247313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247313 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pXYFPN
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PopZ
  • Species
    Caulobacter crescentus
  • Entrez Gene
    popZ (a.k.a. CCNA_01380)
  • Promoter PPopZ
  • Tags / Fusion Proteins
    • sfgfp (N terminal on insert)
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atggtgagcaagggcgaggagg
  • 3′ sequencing primer ctcggggacgcggcgcctaa
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pXYFPN-2-PPopZ-mCherry-sfGFP-PopZ

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLN053 was a gift from Wei Zhao (Addgene plasmid # 247313 ; http://n2t.net/addgene:247313 ; RRID:Addgene_247313)

  • For your References section:

    Scaffold-Scaffold Interaction Facilitates Cell Polarity Development in Caulobacter crescentus. Lu N, Duvall SW, Zhao G, Kowallis KA, Zhang C, Tan W, Sun J, Petitjean HN, Tomares DT, Zhao GP, Childers WS, Zhao W. mBio. 2023 Apr 25;14(2):e0321822. doi: 10.1128/mbio.03218-22. Epub 2023 Mar 27. 10.1128/mbio.03218-22 PubMed 36971555
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