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pNarsenic_noProm
(Plasmid #247321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247321 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTwist+Kan+High
  • Backbone manufacturer
    Twist Bioscience
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    fdeR
  • Species
    Herbaspirillum seropedicae
  • GenBank ID
    WP_013233032.1
  • Promoter P_fdeR

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAAGTGCCATTCCGCCTGAC
  • 3′ sequencing primer GTGGTTATTCACGGTGCCTTCC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mkate2
  • Species
    Synthetic
  • Promoter None

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCACCAACCTCACTTACTCC
  • 3′ sequencing primer CACTGAGCCTCCACCTAGC
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    Arsenical resistance operon repressor
  • Alt name
    arsR
  • Species
    Escherichia coli str. K-12 substr. MG1655
  • GenBank ID
    GCF_000005845.2
  • Promoter P_fdeA

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGAGCCTGGCCATGACAACG
  • 3′ sequencing primer GAAGTGCCATTCCGCCTGAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNarsenic_noProm was a gift from Eveline Peeters (Addgene plasmid # 247321 ; http://n2t.net/addgene:247321 ; RRID:Addgene_247321)

  • For your References section:

    Development and characterization of pNarsenic: a naringenin-inducible biosensor for arsenic in Escherichia coli. Crabbe V, Unal E, De Graeve S, Guerra DG, Peeters T, de Buyl S, Peeters E, Bervoets I. Synth Biol (Oxf). 2026 Jan 10;11(1):ysag001. doi: 10.1093/synbio/ysag001. eCollection 2026. 10.1093/synbio/ysag001 PubMed 41608477
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