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Str-KDEL_CD63-SBP-EGFP
(Plasmid #247324)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247324 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pIRES-neo3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5275
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD63
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    717
  • GenBank ID
    NM_001780.6
  • Entrez Gene
    CD63 (a.k.a. AD1, HOP-26, ME491, MLA1, OMA81H, Pltgp40, TSPAN30)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Streptavidin-KDEL (N terminal on insert)
    • SBP-EGFP (inserted in the small luminal loop of CD63)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Franck Perez, Str-KDEL_CD63-SBP-mCherry (Addgene #222326).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Str-KDEL_CD63-SBP-EGFP was a gift from Franck Perez & Clotilde Thery (Addgene plasmid # 247324 ; http://n2t.net/addgene:247324 ; RRID:Addgene_247324)

  • For your References section:

    Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Mathieu M, Nevo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Thery C. Nat Commun. 2021 Jul 19;12(1):4389. doi: 10.1038/s41467-021-24384-2. 10.1038/s41467-021-24384-2 PubMed 34282141
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