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pEF1-H2B-mRFP1-pcdk1-EGFP1-FRT
(Plasmid #247334)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247334 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA5/FRT& pEF5/FRT/V5- DEST
  • Backbone manufacturer
    Invitrogen; V601020 &Invitrogen; V602020
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B (H2BC11)
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1078
  • Entrez Gene
    H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
  • Promoter EF1α
  • Tag / Fusion Protein
    • mRFP1 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1-H2B-mRFP1-pcdk1-EGFP1-FRT was a gift from Kazuhiro Maeshima (Addgene plasmid # 247334 ; http://n2t.net/addgene:247334 ; RRID:Addgene_247334)

  • For your References section:

    Local nucleosome dynamics facilitate chromatin accessibility in living mammalian cells. Hihara S, Pack CG, Kaizu K, Tani T, Hanafusa T, Nozaki T, Takemoto S, Yoshimi T, Yokota H, Imamoto N, Sako Y, Kinjo M, Takahashi K, Nagai T, Maeshima K. Cell Rep. 2012 Dec 27;2(6):1645-56. doi: 10.1016/j.celrep.2012.11.008. Epub 2012 Dec 13. 10.1016/j.celrep.2012.11.008 PubMed 23246002
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