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pEF1-EGFP-fibrillarin-FRT
(Plasmid #247341)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247341 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEF5
  • Backbone manufacturer
    Invitrogen, Cat#V602020
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fibrillarin (FBL)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1730
  • Entrez Gene
    FBL (a.k.a. FIB, FLRN, Nop1, RNU3IP1)
  • Promoter EF1α
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1-EGFP-fibrillarin-FRT was a gift from Kazuhiro Maeshima (Addgene plasmid # 247341 ; http://n2t.net/addgene:247341 ; RRID:Addgene_247341)

  • For your References section:

    Density imaging of heterochromatin in live cells using orientation-independent-DIC microscopy. Imai R, Nozaki T, Tani T, Kaizu K, Hibino K, Ide S, Tamura S, Takahashi K, Shribak M, Maeshima K. Mol Biol Cell. 2017 Nov 7;28(23):3349-3359. doi: 10.1091/mbc.E17-06-0359. Epub 2017 Aug 23. 10.1091/mbc.E17-06-0359 PubMed 28835378
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