Skip to main content
Addgene
Open Menu

pIx2-mH3.1p-H3.1-EGFP-3′UTR-FRT
(Plasmid #247342)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247342 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pEF5
  • Backbone manufacturer
    Invitrogen, Cat#V602020
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H3.1 (H3C10)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1152
  • Entrez Gene
    H3c10 (a.k.a. H3-291, H3c1, H3c11, H3c8, Hist1h3h)
  • Promoter mH3.1p
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIx2-mH3.1p-H3.1-EGFP-3′UTR-FRT was a gift from Kazuhiro Maeshima (Addgene plasmid # 247342 ; http://n2t.net/addgene:247342 ; RRID:Addgene_247342)

  • For your References section:

    Density imaging of heterochromatin in live cells using orientation-independent-DIC microscopy. Imai R, Nozaki T, Tani T, Kaizu K, Hibino K, Ide S, Tamura S, Takahashi K, Shribak M, Maeshima K. Mol Biol Cell. 2017 Nov 7;28(23):3349-3359. doi: 10.1091/mbc.E17-06-0359. Epub 2017 Aug 23. 10.1091/mbc.E17-06-0359 PubMed 28835378
Related items: