pEF-1α-Venus-Nup107-FRT
(Plasmid
#247344)
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PurposeExpresses Venus-NUP107 under EF1 promoter and can be integrated into FRT site
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247344 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepEF5
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Backbone manufacturerInvitrogen, Cat#V602020
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNup107 (NUP107)
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SpeciesH. sapiens (human)
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Insert Size (bp)3498
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Entrez GeneNUP107 (a.k.a. GAMOS7, NPHS11, NUP84, ODG6, ODG6; GAMOS7)
- Promoter EF1α
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Tag
/ Fusion Protein
- Venus (N terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer T7 or EF1a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-1α-Venus-Nup107-FRT was a gift from Kazuhiro Maeshima (Addgene plasmid # 247344 ; http://n2t.net/addgene:247344 ; RRID:Addgene_247344) -
For your References section:
Single nucleosome imaging reveals loose genome chromatin networks via active RNA polymerase II. Nagashima R, Hibino K, Ashwin SS, Babokhov M, Fujishiro S, Imai R, Nozaki T, Tamura S, Tani T, Kimura H, Shribak M, Kanemaki MT, Sasai M, Maeshima K. J Cell Biol. 2019 Mar 1. pii: jcb.201811090. doi: 10.1083/jcb.201811090. 10.1083/jcb.201811090 PubMed 30824489